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ATP Standard Solution Preparation: ATP powder (1 vial): Store at -20 C.Before use: Add 1.8 mL distilled water to prepare a 1 mol/mL ATP stock solution, then store at -20 C.Dilution series: Dilute the 1 mol/mL ATP stock solution with distilled water to prepare 0.5, 0.1, 0.05, 0.01, and 0.005 mol/mL ATP standard solutions.ADP Standard Solution Preparation: ADP powder (1 vial): Store at -20 C.Before use: Add 2.34 mL distilled water to prepare a 1 mol/mL ADP stock solution, then store at -20 C.Dilution series: Dilute the 1 mol/mL ADP stock solution with distilled water to prepare 0.5, 0.1, 0.05, 0.01, and 0.005 mol/mL ADP standard solutions.Tissue Homogenization: Weigh 0.3 g tissue sample, add 1.5 mL Extraction Buffer I, and homogenize in an ice bath

Understanding the Master Antioxidant Glutathione is a tripeptide, which is a small protein-like molecule made of three amino acids: cysteine, glutamic acid, and glycine

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