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The further steps included incubation of specimens in 2% osmium tetroxide (Sigma-Aldrich) for 2 h at room temperature and dehydration in a series of ethanol solutions (Linegal Chemicals, Poland), starting from 10 min twice in 50 and 70%, followed by 5 min twice in 95% and 1 min twice in 100% ethanol solution

Urdaneta KE, Castillo MA, Montiel N, Semprn-Hernndez N, Antonucci N, Siniscalco D (2018) Autism spectrum disorders: potential neuro-psychopharmacotherapeutic plant-based drugs

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These include images captured from the same experiment (with no Alexa Fluor 488 secondary to detect total GSTP1) but in the absence of -alkynyl-palmitate (Fig 2B.i) and the click chemistry step (Fig 2B.ii)