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These findings demonstrate that palmatine alleviates MASLD by modulating a novel target network to inhibit hepatocyte apoptosis, providing a robust, hypothesis-driven preclinical foundation for its therapeutic development

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A 34-min linear gradient (375% acetonitrile in 0.1% formic acid), at a flow rate of 350 nL/min, was used to separate peptides on polaris-HR-Chip C18 column (150 mm long 75 m inner diameter)

This was useful as a reductionist measure, because it allowed him to analyze the initiation and promotion periods separately