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Because T cell subsets with low differentiation (such as T N , T CM and T SCM ) have strong self-renewal ability, CAR-T cells products with high contents of T N , T CM and T SCM cells show excellent antitumor responses and persistence in vivo ( 2.1 Nave T cells (Tn) Nave T cells(T n )remain in a quiescent state, with low metabolic demands, and use predominantly mitochondrial oxidative phosphorylation (OXPHOS) fueled by small amounts of glucose, amino acids and fatty acids to generate ATP ( via tonic TCR ( in vitro study suggested that nave T cells from patients with metastatic melanoma exhibit increased transgene expression and proliferation ( 2.2 Effector T cells (Teff/Treg) Upon activation, T cells rapidly engage in multiple metabolic pathways to support their growth, proliferation, and effector function