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A large mixed vial may look convenient, but if the protocol only uses a small amount each week, the later withdrawals happen when the solution is older and has been punctured more times

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Thijssen DH, Black MA, Pyke KE, Padilla J, Atkinson G, Harris RA, Parker B, Widlansky ME, Tschakovsky ME, Green DJ (2011) Assessment of flow-mediated dilation in humans: a methodological and physiological guideline

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