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Animal procedures were approved by the Ethics Committee for Animal Welfare of the University of the Basque Country UPV/EHU (CEEA 401/2015), CIMUS, University of Santiago de Compostela-Instituto de Investigacin Sanitaria (15010/17/007) and Centro Nacional de Investigaciones Cardiovasculares (CNIC) (PROEX 215/18) and were conducted in conformity with the EU Directives for animal experimentation