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STEP 4: Once you have managed to shape your filter, it is time to place it in its corresponding place with care and delicacy

This method is completely safe, inexpensive and works well against food containers, kitchenware and other types of household plastics

RT-qPCR RNA isolation, Primer Sequences, RT-PCR on Tfam, OPA1, Drp1, Fis1, Mfn1, Mfn2 using MyiQ single-color Real-Time thermal cycler [30] and RT-PCR for IL-6, IL-8, IL-1, Mn-SOD, PINK1, PPARGC1, OPA1 and TFAM using Taqman were performed as described in the online data Additional file 1

When non-smokers are exposed to secondhand smoke it is called involuntary smoking or passive smoking