Herpesvirus screening Viral DNA was extracted (Stratec Biomedical, Germany) from collected horse samples using a commercially available kit (QIAamp DNA Blood Mini Kit, Giagen) as described previously 40,41,42,43
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Areas requiring Focus / Improvement Address the immediate recruitment and retention pressures being faced in relevant areas of Community Protection, thus ensuring that statutory duties can be delivered on an on-going basis Provide on-going support to staff in relation to the implementation of any new systems being introduced corporately make sure that service delivery is running as effectively as possible Review Community Protection material for the Council website to ensure that all content is up to date, accurate, complete and in line with the citizen-centric approach Collate and coordinate the agreed actions of the approved Local Child Poverty Act Report Review Shared Prosperity funding programmes for 2025 Update and refresh Community Grant Scheme workshop content Provide ongoing support to staff in relation to the implementation of new internal systems and implementations of national strategies and revised legislation Attendance at new 2-weekly Community Harm and Risk Meeting (CHARM) with Police Scotland, Community Safety, Trading Standards and Licensing officers continues

RT-PCR Total cell RNA extraction was performed using TRIzol (Invitrogen) and reverse transcribed according to the manufacturers protocol (Takara)