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Quantitation of serum nicotine, cotinine, adrenalin, and -endorphin via Ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) Nicotine and cotinine This study used a validated SHIMADZU UFLC-MS/MS system (Waters Corporation, Milford, MA, USA) to determine the concentration of nicotine and cotinine in rat serum

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At extreme levels, it can be toxic to humans following inhalation, oral or dermal exposures, according to the US Environmental Protection Agency

Even persons who have smoked for many years can experience substantial health and financial benefits if they stop smoking