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TPO-deficient cultures were maintained in StemSpan SFEM II medium supplemented with IL-6, IL-9, and SCF (10 ng/mL each), supplemented without or with TPO 10 ng/mL, and 10 M BCT1028 (or v/v DMSO as control) every 24 h

doi: 10.1038/s41598-025-96746-5 65 CheCSunXWuYMaLHuYYangWet al

8991 As a net effect, the combination of high sensitivity of CD8 effector T cells with resistance of immunosuppressive Treg cells leads to suppression of anti-tumor immune response

Given that this spatial arrangement and physicochemical properties of the cargo are unique to cyanobacteria, there may not be a requirement for a comparable protein in other bacterial secretin complexes