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(2015) and Mischley et al

The samples were subjected to click reaction by adding 5 l biotin azide (final: 100 M), 25 l BTTAA (final: 500 M), 10 l CuSO 4 (final: 1 mM) and 10 l NaAsc (final: 2 mM) to a total volume of 500 l and incubated for 1 h at r.t