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For this experiment, the temperature was maintained at 60 C, as OsDHAR and catalase (CAT) aggregate at this temperature over time (Fig

Females resilient to trauma exhibited higher pre-trauma levels of Roseburia, Oscillibacter , and Lachnospiraceae , suggesting that baseline enrichment of SCFA-producing bacteria may confer resistance to PTSD onset (Tanelian et al., 2023)

At the metabolite level, changes were predominant in the catabolism of FFAs and branch-chain amino acids (BCAAs), mitochondrial/peroxisomal and proteasomal protein processing, and the biosynthesis of acetyl-CoA, acyl-CoAs and purine nucleotide bisphosphates

Thereafter, anhydrous sodium sulfate was added to remove moisture from the product which was then filtered and distilled under reduced pressure to remove ethyl acetate, followed by fractional distillation under reduced pressure (93-95 C/5 mmHg) to obtain 40.4 g (yield: 80 %) of ethyl 4-chloro-3-hydroxybutyrate