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Biochemical and histological analyses were performed on the hippocampal and cortical tissue, assessing oxidative stress markers, antioxidant enzyme activity, and glial activation through spectrophotometric assays and immunohistochemistry

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Neurones are highly specialised cells with limited self-protective capabilities and contribute little to adaptive nervous tissue responses to damage

The mechanisms likely involve immune modulation (microglia in ALS can adopt a neurodegenerative phenotype that might be restrained by microbial signals) and metabolic support (providing nutrients like nicotinamide or SCFAs to neurons and glia) [214, 218]