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Data availability Sequence data that support the findings of this study have been deposited in the Code availability All authors have access to the codes used in the study and take responsibility for the integrity and accuracy of the analyses

While the European Union introduced new regulations in May 2016 according to which nicotine levels are not allowed to exceed a dose of more than 20 mg/ml (28), baseline data were acquired between July and September 2016 and e-cigarette users might still have had liquid containers with higher nicotine levels stored at home, or might have mixed their own liquids

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