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After 4 h of 37 C MTT incubation, each well received 100 L of 10% SDS in 0.01 M HCl and was maintained in a humidified incubator for the whole night

Therefore, while the antioxidant activity of catechol estrogens may partially contribute to their protective effect against chemically-induced ferroptosis, its overall contribution likely is relatively small

A cross-sectional survey of 38 ME/CFS patients receiving long-term B12 injections and oral folic acid demonstrated that higher injection frequencies and doses, combined with elevated daily folic acid intake, were associated with improved clinical outcomes [126]

Notably, all reported mutations in NFE2L2 were gain-of-function, and 40% in KEAP1 were loss-of-function, suggesting that constitutive antioxidant pathway stimulation is protective against ionizing radiation